Precooled methanol-water (80%, vol/vol) was added to the cell pellets, and the cells were scraped off with a cell scraper into a 2-mL centrifuge tube with ring- d 5 -labeled-phenylalanine added as an internal standard
Chapter 4 | - If It Could Happen to Me, What Will Happen to You
Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions
His chronic anemia resolved without any iron or blood products, with a hemoglobin increase from 11.7 to 14.7
Corn, C