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In this study, our results suggested that the core targets among riboflavin, osteogenesis and osteoclastogenesis included HIF1A, HDAC2/3/4, TP53, PPARG, PPARGC1A, NFKB1, MYC and BCL2, and the potential signaling pathways of vitamin B2 involved in regulating bone health focused on HIF-1 signaling pathway, p53 signaling pathway, AGE-RAGE signaling pathway, longevity regulating pathway, and so on