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The conserved lysine residue N-terminal to the active site, Cys A , helix 1 and helix 3 as well as the -sheets of the thioredoxin fold are almost perfectly superimposed

A 12.5 gram pellet of cells was resuspended in 37.5 ml buffer T (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.5 mM EDTA, 2 mM 2-Mercaptoethanol, 0.01% Igepal CA-630) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (2 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed with a Dounce homogenizer and brief sonication

This unique design maximises the effectiveness of GHK-Cu by delivering it consistently to the scalp, promoting healthier, stronger hair growth