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Hum Genet 123(4):321332 Heisterkamp N, Rajpert-De Meyts E, Uribe L, Forman HJ, Groffen J (1991) Identification of a human gamma-glutamyl cleaving enzyme related to, but distinct from, gamma-glutamyl transpeptidase

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Briefly, equal amount of samples were loaded onto 12% SDSPAGE gels, transferred to polyvinylidene difluoride (PVDF) membranes and analyzed using antibody against GPX4 at a concentration of 1:2,000