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Western Blot HepG2 cells were homogenized on ice with RIPA buffer supplemented with phosphatase and protease inhibitors (Pierce, Thermo Scientific, MA, USA)

Life Sci 2001

tuberose methanolic root extract (PTME) as a potential herbal treatment on the liver homogenates of alloxan-induced diabetic rats, it was reported that PTME possessed an abundance of flavonoids and significantly reduced DPP-IV activity in a time-dependent manner from 16 DPP-IV activity in a time-dependent manner fromto 4 M/unit as compared with alloxan control (17.5 M/unit) within a period of 40 days, which proved that PTME was a promising candidate to cure DPP-IV-induced liver disorders ( in vitro results also supported the data as a potential DPP-IV inhibitor by exhibiting a considerable IC 50 value of 17.4 mg/ml

One calculation error can mean injecting double or half your intended dose

The limitations relative to Retatrutide are real, though