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The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual

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There are a couple of local businesses that say that the business is better [in the past four years], but theres no evidence of that, the mayor said