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Retrieved July 26, 2014

After washing twice in 1 PBS, cells were resuspended in ALI expansion medium with RI and seeded onto bovine collagen-coated 6.5 mm transwell inserts (2 10 4 cells/insert) containing ALI Expansion medium with RI in the basolateral chamber (day 5)

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In the first four decades of the 20th century the uses of shadows were important compositional elements, especially in portraiture and theater and dance photography