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Sister-Chromatid Exchange Analyses in Peripheral Blood Lymphocytes For the SCE analysis in PBLs, cultures were established in duplicate as described previously ( Micronucleus Analysis in Buccal Exfoliated Epithelial Cells Buccal exfoliated epithelial cells were smeared onto slides, air-dried and fixed in 80% cold methanol, for 20 min (59) without cytoplasm counterstain

Crafted using organic tobacco, this variant stands out for its smoother taste compared to other American Spirit options

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Nicotiana rustica : Mostly known as Wild Tobacco, this variety of tobacco typically contains up to nine times more nicotine than other varieties of tobacco