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Then, through GC-MS in tandem mode (GC-MS-MS) it was made possible to identify the presence of pesticides and polycyclic aromatic hydrocarbons (PAHs)

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Finally, qRT-PCR reactions were performed in triplicates with SYBR Select Master Mix (Thermo Scientific) in an ABI7500 Fast (Applied Biosystems) real-time qRT-PCR system