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[25] Excess nicotinic acid is methylated in the liver to N 1 -methylnicotinamide (NMN) and excreted in urine as such or as the oxidized metabolites N 1 -methyl-2-pyridone-5-carboxamide and N1-Methyl-4-pyridone-3-carboxamide (2PY and 4PY)

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The upregulation of these metabolites may reflect a physiological response in tobacco to enhance its resistance to chilling stress by strengthening antioxidant and defense mechanisms