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The MS/MS data were processed and subjected to database searches by using MASCOT Daemon (Matrixscience)

Two experts in tobacco and nicotine addiction (JR and KF) and two experts in self-report measure development (TS and SC) reviewed the proposed conceptualization and contributed to the development of the first draft measure, which included nine items that characterized the degree of perceived dependence on three different response scales (intensity, frequency, or duration) adapted to the characteristics of the individual symptoms (see Supplementary Table S2)

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