Western blotting Cells were subjected to western blot analysis as previously reported [22] using the following antibodies: Nrf2 (PA5-27882, Invitrogen, RRID:AB_2545358), Keap1 (8407, Cell Signaling, RRID:AB_10860776), NF-B P65 (sc-8008, Santa Cruz Biotechnology, RRID:AB_628017), Gpx2 (MA5-36260, Invitrogen, RRID:AB_2890391), Nqo1 (ZRB1707, Millipore Sigma, RRID:AB_3662860), -actin (sc-81178, Santa Cruz Biotechnology, RRID:AB_2223230)
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Among these, miR - 132 is upregulated in the serum of smokers compared with its expression in the nonsmoker controls, and the miR - 132 expression level was positively correlated with the levels of inflammatory cytokines ( IL - 1 and TNF - )
