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Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.83.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol

Clin Endocrinol (Oxf) 44 : 501514 Unterman TG (1993) Insulin-like growth factor binding protein-1: identification, purification, and regulation in fetal and adult life

At 2, 12, 18 and 24 hours media supernatants was analyzed

GHK-Cu GHK-Cu is a copper-binding tripeptide studied extensively for its role in collagen synthesis, skin regeneration, wound healing, and extracellular matrix remodelling

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