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Leos-Toro et al., 2018

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To further assess the effect of CSE on epithelial barrier function, we treated the A549 cell line, the RPMI2650 human nasal epithelial cell line, the BEAS-2B human bronchial epithelial cell line, and the MLE12 mouse lung epithelial cell line with CSE and measured their transepithelial electrical resistance (TEER)

Negative Mood Effects: While some individuals often use tobacco to alleviate stress or improve mood temporarily, long-term tobacco use leads to mood disturbances and irritability, particularly during periods of nicotine withdrawal

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