The isolated precursors were fragmented by collision induced dissociation (CID) at 30% collision energy with the maximum injection time of 35 ms for 3 s (top speed setting) and detected in the ion trap, followed by multinotch (simultaneous) isolation of the top 10 MS2 fragment ions within the m/z range 4001,400, fragmentation (MS3) by higher-energy collision dissociation (HCD) at 55% collision energy and detection in the Orbitrap at 50,000 resolution m/z range 100500 and maximum injection time 105 ms
ntrc extracts contained two immunoreactive GSAAT bands a partially oxidized species and a dominant reduced form while wild-type leaves contained only the reduced from of GSAAT, regardless of whether dark-exposed leaf samples were analyzed after a D to NL (D-NL) or a D to HL (D-HL) transition, or light-exposed samples had undergone a HL-D transition ( Figure 3C )
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Supplementary Equation 1), and (RPE sensitivity, often referred to as learning rate
