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When a pigmented tumor is detected in internal organs or mucous membranes, the metastatic nature of the lesion should be excluded

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Here we use urinary extracellular vesicles (EVs), RNA sequencing, droplet digital PCR, and predictive modeling to identify downregulation of metabolism transcripts Phosphoenolpyruvate carboxykinase-1 , 4-Hydroxyphenylpyruvate dioxygenase , Dihydropyrimidinase , Glutathione S-transferase alpha-1 , Aminoacylase-1 , and Electron transfer flavoprotein B in DM1